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YIplac204-iGFP-YPT52

Plasmid

#105279

Purpose

Plasmid for integration of iGFP-Ypt52 at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt52 (YPT52 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB3647

Plasmid

#80981

Purpose

Used for abrB3647(CAC3647) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB3647(CAC3647)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB1941

Plasmid

#80980

Purpose

Used for abrB1941(CAC1941) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB1941(CAC1941)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB0310

Plasmid

#80979

Purpose

Used for abrB0310(CAC0310) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB0310(CAC0310)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pALBRSF12

Plasmid

#46376

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein, inactive

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD25-74

Plasmid

#46375

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

TvoVMA intein

Tags

H6-GB1 and SH3

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a207-479.wt

Plasmid

#17516

Depositor

David McKay

Article

Wang et al Biophys J. 2008 Jan 1. 94(1):L01-3.

Insert

B. subtilis DEAD-box helicase YxiN second domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 1-206

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al bioRxiv 2023.09.08.556841

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry_a_tubulin_IRES_puro2

Plasmid

#21043

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl

Plasmid

#90485

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-mCherry2Bx6

Plasmid

#105267

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Tags

mEGFP

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

msCFPx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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