We narrowed to 15,158 results for: pET

1. pET-His6-Sumo-CfaC-MBP

   Plasmid

   #126018

   Purpose

   Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

   Depositor

   Thomas Muir

   Insert

   His6-Sumo-CfaC-MBP
   

   Tags

   6xHis-SUMO and HA-MBP

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-mTagBFP2-TEV-Strep-His

   Plasmid

   #210824

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mTagBFP2
   

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-Tev-Keap1

   Plasmid

   #62456

   Purpose

   His6-halo tagged human Keap1 protein for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Halo-Tev

   Expression

   Bacterial

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-15b-hAR-663-919

   Plasmid

   #89083

   Purpose

   bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

   Insert

   hAR ligand binding domain (AR Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 663-919

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 E2 LBD-Tev-E1a-His

   Plasmid

   #232117

   Purpose

   For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   DBT (DBT Human)
   

   Tags

   6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+) NRP1 b1b2 (273-586)

   Plasmid

   #162733

   Purpose

   Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1b2 domains (residues 273-586)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   March 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET28a-GGG-ELP(120nm)-Cys

   Plasmid

   #91572

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(120nm)-Cys
   

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET co-transformation cloning vector (13S-A)

   Plasmid

   #48323

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204403

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid

    #101239

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b-HOXD13(+7A)-IDR-mCherry

    Plasmid

    #145281

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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