We narrowed to 41,903 results for: tro

1. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_CMV_MeltNLS/NES-40-mCherry

   Plasmid

   #234066

   Purpose

   Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltNLS/NES-40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tg (mpx:mCherry-2A-Rac2D57N)

   Plasmid

   #58934

   Purpose

   expresses dominant negative rac2 tagged with mcherry in neutrophils

   Depositor

   Anna Huttenlocher

   Article

   Deng et al Dev Cell. 2011 Oct 18;21(4):735-45. doi:

   Insert

   mcherry-2a-Rac2D57N (rac2 Zebrafish)
   

   Use

   Tags

   mcherry2a

   Expression

   Mutation

   changed Aspartate 57 to Asparagine

   Promoter

   mpx

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-PTE2A

   Plasmid

   #111773

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.PARP1#1

   Plasmid

   #14544

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLV.PARP1#2

   Plasmid

   #14545

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pHEFI-OptoCARCD19-mScarlet

   Plasmid

   #170465

   Purpose

   Expresses an optically-controllable Chimeric antigen receptor that binds CD19 antigen, fused to mScarlet fluorophore

   Depositor

   John James

   Article

   Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

   Insert

   OptoCAR-CD19
   

   Use

   Lentiviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-krt5

   Plasmid

   #82585

   Purpose

   5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

   Depositor

   Kryn Stankunas

   Article

   Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

   Insert

   zebrafish krt5 promoter (krt5 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-FLIP-puro-dsRed-GFP-miRNA

   Plasmid

   #32704

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   emGFP-miRNA
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG3 N

    Plasmid

    #132941

    Purpose

    MLV packaging plasmid for N-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    N-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-3xFLAG-TEV-UBE2O

    Plasmid

    #105718

    Purpose

    Expresses WT full length human N- terminally tagged UBE2O

    Depositor

    Ramanujan Hegde

    Article

    Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

    Insert

    UBE2O (UBE2O Human)
    

    Use

    Tags

    3xFLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-OptoCAR-mScarlet

    Plasmid

    #170463

    Purpose

    Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

    Depositor

    John James

    Article

    Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

    Insert

    OptoCAR
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLOX-Ttag-iresTK

    Plasmid

    #12246

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    SV40 Large T, HSV1-TK (SV40gp6 Simian virus)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORF1-2

    Plasmid

    #206024

    Purpose

    An expression vector for mouse LINE1 ORF1-2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1-2 derived from mouse LINE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS253

    Plasmid

    #114271

    Purpose

    SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

    Insert

    Cbr-Unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    *please see depositor comment below

    Promoter

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-YFP-PKD1

    Plasmid

    #31527

    Depositor

    Alexandra Newton

    Article

    Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

    Insert

    YFP-HA-Protein Kinase D (PRKD1 Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGL0_20 [panARS-opt ORI]

    Plasmid

    #198933

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    panARS-opt ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ILID-CAAX

    Plasmid

    #85680

    Purpose

    Expression of untagged ILID CAAX

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    ILID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ centrin-mKate2

    Plasmid

    #105941

    Purpose

    in vitro transcription, zf centrin N-terminally tagged with mkate2

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Centrin
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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