We narrowed to 4,320 results for: Pet2;

1. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

   Plasmid

   #181744

   Purpose

   bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY-HF1
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

   Promoter

   T7

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

   Plasmid

   #171586

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS-HA

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   His-MBP-NLS-RfxCas13d-NLS-HA
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6xHis-MBP-TEV site-TadA8e

   Plasmid

   #208319

   Purpose

   Overxpression MBP-TadA8e

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8e
   

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

   Plasmid

   #235150

   Purpose

   Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   HaloTag linked to SpyCatcher3
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a human Tankyrase2 full length Y920E

   Plasmid

   #132639

   Purpose

   expresses human Tankyrase2 full length Y920E in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

   Insert

   Tankyrase2 Y920E
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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