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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 170,876 results for: addgene

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Showing: 3721 - 3740 of 170876 results

  1. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  3. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  7. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    ARG8
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

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  8. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    GNP1
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

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  16. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  17. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  18. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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Showing: 3721 - 3740 of 170876 results