We narrowed to 13,740 results for: TIM;

1. GFP-LiMETER-v1

   Plasmid

   #113933

   Purpose

   Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   LOV2 and Rit polybasic domain (PB)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMY-IRES-eGFP

   Plasmid

   #163361

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule2-Acd82

   Plasmid

   #174100

   Purpose

   Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   Acridone 82 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP gRNA (BRDN0000561167)

   Plasmid

   #80034

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. C1A-C1A-EYFP

   Plasmid

   #61155

   Purpose

   Diacylglycerol (DAG) reporter

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C1A from PKCG amino acids 26-89

   Promoter

   CMV

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SWEET11:SWEET11-2A-GFP-GUS

   Plasmid

   #172484

   Purpose

   Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

   Insert

   SWEET11 (AT3G48740 Mustard Weed)
   

   Use

   Tags

   GFP, GUS

   Expression

   Plant

   Mutation

   Promoter

   SWEET11

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti F9-TetR-EGFP-IRES-PuroR

   Plasmid

   #117049

   Purpose

   lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Insert

   F9-TetR-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   F9 promoter

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEGFP-HRas S17N

   Plasmid

   #18665

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   S17N mutation in H-Ras

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223-MCU-D261A

    Plasmid

    #31729

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed D261 to Alanine

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK Tgfbr3-HA neo

    Plasmid

    #58508

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Dominant negative Hsp90 beta

    Plasmid

    #22480

    Depositor

    William Sessa

    Article

    Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

    Insert

    Dominant negative Hsp90 (HSP90AB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-MCU-E264A

    Plasmid

    #31730

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed E264 to Alanine

    Promoter

    None

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK-Drosha(UniProt)-FLAG-mEGFP

    Plasmid

    #164691

    Purpose

    Express Drosha-FLAG-mEGFP in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    Drosha (DROSHA Human)
    

    Use

    Tags

    FLAG and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-MCU-E257A

    Plasmid

    #31727

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Changed E257 to Alanine

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLNCX2-mCherry-CHMP2A

    Plasmid

    #115330

    Purpose

    expresses mCherry-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lck-mTurquoise2-iLID

    Plasmid

    #176125

    Purpose

    Plasma membrane localized iLID

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE:mTREK-1(tandem wt-wt)

    Plasmid

    #224757

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

    Depositor

    Dan Minor

    Article

    Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

    Insert

    Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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