Addgene

We narrowed to 1,961 results for: IPP

Showing: 541 - 570 of 1961 results

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pEF-mRuby3

Plasmid

#179730

Purpose

Mammalian expression of mRuby3 from the EF1 promoter

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-HaloTag

Plasmid

#188907

Purpose

Expresses synaptic HaloTag

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

SYP (Syp Mouse)

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHACK(Gal4)-DONR(T2A-LexAGAD)

Plasmid

#194769

Purpose

To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-LexAGAD

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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ZipGFP1-10_TEV

Plasmid

#81242

Purpose

Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:WTIP

Plasmid

#108231

Purpose

Lentiviral vector for Tet-inducible WTIP expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

WTIP (WTIP Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tet/ON Minimal CMV promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHACK(Gal4)-DONR(T2A-Flp1)

Plasmid

#194770

Purpose

To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-Flp1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-myr-GFP

Plasmid

#83468

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Myristoylated GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

doxycycline-inducible promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEF mito-cGFP (matrix)

Plasmid

#188904

Purpose

Expresses mitochondrial GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 S6K2 WT

Plasmid

#17729

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 Y287A

Plasmid

#32824

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E5D, Y287A

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pNICE-LAP-neurexin-1beta

Plasmid

#42575

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LAP-neurexin-1beta

Use

Tags

c-Myc, after signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD4d-U6-1:white1-U6-3:white1

Plasmid

#84005

Purpose

pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Inserts

white sgRNA-1
white sgRNA-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6:1, U6:3, and dU6-1; dU6-3

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBS_hNFATc4nuc

Plasmid

#24586

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

NFATc4 (NFATC4 Human)

Use

Cloning vector

Tags

EE tag

Expression

Mutation

S167A, S169A, S172A, S173A, S174A, S213A, S217A, …

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2-GFP1-9

Plasmid

#124433

Purpose

Expresses GFP1-9 in zebrafish

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

mutant Odd Skipped

Use

Tags

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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XW52

Plasmid

#65838

Purpose

XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Czipper-QF-AD-NLS

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pmig-13

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242A

Plasmid

#32823

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242A

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
CMV500 A-SREBP2

Plasmid

#33358

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Insert

SREBP2

Use

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pF(UG) CamKII sf iGluSnFR

Plasmid

#158769

Purpose

iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

iGluSnFR

Use

Lentiviral

Tags

Expression

Mutation

Additional ER and Golgi export sequences

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-4c

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pShGSK3bE

Plasmid

#37526

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

GSK3b (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SRa promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-CB2gRNA-CBh-mCherry

Plasmid

#91948

Purpose

Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-TRP1

Plasmid

#41603

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST and yeast ADH1 terminator

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCIneoFH human RASSF6

Plasmid

#40344

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF6 (RASSF6 Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pClneoFLAG human RASSF4

Plasmid

#37020

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

rassf4 (RASSF4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only