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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,168 results for: ena

Showing: 721 - 760 of 11168 results

  6. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Use
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

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  7. pPKm-232

    Plasmid
    #90501
    Purpose
    pSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoter
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-tHO1 and mito-tPCYA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    90501-plasmid-map-sequence-id-204669

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  8. pPKm-234

    Plasmid
    #90507
    Purpose
    pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-sHO1, mito-sPCYA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    90507-plasmid-map-sequence-id-204697

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  9. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

    Plasmid
    #225440
    Purpose
    Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.
    Depositor
    Diego Orzaez
    Insert
    nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225440-plasmid-map-sequence-id-447732

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  10. pCOA-Leu-FvPPT1

    Plasmid
    #179923
    Purpose
    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Insert
    FvPPT1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179923-plasmid-map-sequence-id-359550

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  11. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Wolfgang Kroutil
    Article
    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185456-plasmid-map-sequence-id-367933

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  16. pPKm-240

    Plasmid
    #90510
    Purpose
    pSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    cyto-sFD, cyto-sFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    90510-plasmid-map-sequence-id-204691

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  18. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164558-plasmid-map-sequence-id-330194

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  22. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  23. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

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  36. MtPT4-p3

    Plasmid
    #160003
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160003-plasmid-map-sequence-id-323050

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  38. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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Showing: 721 - 760 of 11168 results