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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-T19N

Plasmid

#252259

Purpose

Express HA-RHOA(T19N)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA T19N

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-Y42C

Plasmid

#252261

Purpose

Express HA-RHOA(Y42C)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA Y42C

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-EGFP-pA

Plasmid

#200520

Purpose

Mini circle middle entry plasmid for membrane GFP- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

memb-GFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-mCherry-pA

Plasmid

#200528

Purpose

mini circle middle entry plasmid for membrane mCherry-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-BFP-pA

Plasmid

#200537

Purpose

mini circle middle entry plasmid for membrane BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-Membrane

Plasmid

#168832

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-Membrane (M )

Use

Gateway Cloning

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-Membrane

Plasmid

#168966

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Membrane

Plasmid

#168925

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Membrane (HCNV63gp5 )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Membrane

Plasmid

#168946

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Membrane

Plasmid

#168905

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-Membrane

Plasmid

#168880

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-Membrane

Plasmid

#168840

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa-C3

Plasmid

#185587

Purpose

to recruit Vasa-C3 to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-C3

Use

In vitro transcription (mrna synthesis)

Tags

mCherry and membrane targeting sequence from Neur…

Mutation

C3 mutant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa

Plasmid

#185586

Purpose

to recruit Vasa to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry, S-Tag and membrane targeting sequence fr…

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Mutation

K82R kinase-dead mutant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled Library

#83994

Purpose

Mouse CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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