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pBT346.6-H3.3-K4M-bGHpolyA

Plasmid

#128743

Purpose

Inducible expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

K4M

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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YIp204-P30-His-POL30

Plasmid

#99550

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ306 (GLT1 POL1 24xPP7SL)

Plasmid

#35196

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

GLT1 POL1 24xPP7SL

Available Since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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KZ701: pMVP (L3-L2) AID + polyA

Plasmid

#121791

Purpose

pMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

AID + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

Plasmid

#112287

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Tyrosine 357 to Phenyalanine corresponding to tyr…

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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DGP104- pSP64 Poly(A)700

Plasmid

#70098

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

700bp insert from B. subtilis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-AN-HA_ WT POLH_deltaGG Ubiquitin

Plasmid

#246411

Purpose

Expresses HA-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH_deltaGG NEDD8

Plasmid

#246412

Purpose

Expresses HA-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_L704A_F707A_F708A (PIP2*) POLH

Plasmid

#246283

Purpose

Expresses L704A_F707A_F708A POLH with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH L704A_F707A_F708A. Coding sequence has been …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG NEDD8

Plasmid

#221870

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG NEDD8

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_D652A POLH

Plasmid

#221871

Purpose

Expresses D652A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

D652A

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_K682A_K686A_K694A_K709A POLH

Plasmid

#221872

Purpose

Expresses K682A_K686A_K694A_K709A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682A, K686A, K694A, K709A

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_L704A_F707A_F708A POLH

Plasmid

#221873

Purpose

Expresses PIP box-mutated POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

L704A_F707A_F708A

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG NEDD8

Plasmid

#221860

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin

Plasmid

#221861

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG Ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_WT POLH

Plasmid

#221868

Purpose

Expresses WT POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin

Plasmid

#221869

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polb(WT)-C-Hygro

Plasmid

#216777

Purpose

Flag-Polb(WT), C-terminal domain (AA 91-335) & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335)

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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