We narrowed to 9,215 results for: Pol

1. 3'dmDR-CMV-CasRx-bGHpolyA

   Plasmid

   #229556

   Purpose

   Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

   Depositor

   Omar Akbari

   Insert

   CasRx
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1203A-CMV-5’DR-EGFP-bGHpolyA

   Plasmid

   #221452

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1203B-CMV-3’DR-EGFP-bGHpolyA

   Plasmid

   #221453

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

   Plasmid

   #221454

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203Q-5'dmDR-NT(22)-GFP-bGHpolyA

   Plasmid

   #221538

   Purpose

   EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA

   Plasmid

   #221539

   Purpose

   EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1203M-CMV-dDiCas7-11-bGHpolyA

   Plasmid

   #220957

   Purpose

   Deactivated DiCas7-11 effector with CMV promoter/enhancer

   Depositor

   Omar Akbari

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1203P-CMV-Cas7.1-bGHpolyA

   Plasmid

   #220960

   Purpose

   Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector)

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag-Jarid2-polyA

   Plasmid

   #223811

   Purpose

   IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

   Depositor

   Azusa Inoue

   Article

   Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

   Insert

   Jarid2 (Jarid2 Mouse)
   

   Use

   In vitro transcription

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDest-pol2-Smarcd1-Myc

    Plasmid

    #227716

    Purpose

    Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

    Depositor

    Kent Hunter

    Article

    Ross et al bioRxiv 2024.01.24.577061

    Insert

    Smarcd1 (Smarcd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    pol2

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2pA ins:Apollo-NADP+ mVenus

    Plasmid

    #210518

    Purpose

    Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

    Depositor

    Jonathan Rocheleau

    Article

    Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

    Insert

    Apollo-NADP+
    

    Use

    Zebrafish expression, tol2 kit gateway-compatible

    Tags

    mVenus

    Promoter

    Zebrafish insulin promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-HA_aa 1-449 POLH

    Plasmid

    #201583

    Purpose

    Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    POLH (POLH Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-449 of DNA polymerase eta

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid

    #201585

    Purpose

    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-542 of DNA polymerase kap…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid

    #192582

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid

    #192581

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST tol2 UbB polyA

    Plasmid

    #188701

    Purpose

    Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T3-GST-PolB(K72A)

    Plasmid

    #177132

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1C-FlucV5His6-PolyA-HhR_AB

    Plasmid

    #148303

    Purpose

    Insect Expression of PolyA-HhR

    Depositor

    Elisa Izaurralde

    Insert

    PolyA-HhR
    

    Expression

    Insect

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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