We narrowed to 9,068 results for: Pol

1. CaMKII-somBiPOLES-ChroME2s-mCerulean

   Plasmid

   #192582

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaMKII-somBiPOLES-ChroME2f-mCerulean

   Plasmid

   #192581

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX4T3-GST-PolB(T304I)

   Plasmid

   #177133

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST tol2 UbB polyA

   Plasmid

   #188701

   Purpose

   Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T3-GST-PolB(K72A)

   Plasmid

   #177132

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1C-FlucV5His6-PolyA-HhR_AB

   Plasmid

   #148303

   Purpose

   Insect Expression of PolyA-HhR

   Depositor

   Elisa Izaurralde

   Insert

   PolyA-HhR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1026L=U6-3-gRNA#1[Pol-γ35]

   Plasmid

   #159674

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#1[Pol-γ35]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1026M=U6-3-gRNA#2[Pol-γ35]

   Plasmid

   #159675

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#2[Pol-γ35]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC_cherry donor_Polb target (pE038)

   Plasmid

   #149349

   Purpose

   Replace donor vector for cherry insertion using the human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   cherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid

    #131241

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert Pol iota se…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_1-590 POLI

    Plasmid

    #131233

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IY001: pMVP (L3-L2) BioID2 + polyA

    Plasmid

    #121795

    Purpose

    pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    BioID2 + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IY202: pMVP (L3-L2) APEX2 + polyA

    Plasmid

    #121796

    Purpose

    pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LC601: pMVP (L3-L2) eYFP + polyA

    Plasmid

    #121769

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LC701: pMVP (L3-L2) eCFP + polyA

    Plasmid

    #121767

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eCFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL3.7-EF-EYFP-YAP1_R89A-PolyA

    Plasmid

    #112291

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Arginine 89 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7-EF-EYFP-YAP1_L91A-PolyA

    Plasmid

    #112292

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Leucine 91 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL3.7-EF-EYFP-YAP1_F95A-PolyA

    Plasmid

    #112293

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Phenylalanine 95 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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