We narrowed to 4,210 results for: chm

1. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti.Cas9.BFP.Blast

   Plasmid

   #196714

   Purpose

   WT SpCas9 with BSD-TagBFP for selection and sorting

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TNFR2-YFP

   Plasmid

   #111210

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   TNFRSF1B (TNFRSF1B Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   S175P, D306G

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cul1-EYFP

   Plasmid

   #111214

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   CUL1 (CUL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW4.04

   Plasmid

   #171209

   Purpose

   bacterial expression of MBP-tagged PGL-3

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   pgl-3 (pgl-3 Nematode)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK19pA-MN

   Plasmid

   #86973

   Purpose

   ChIC; genomic mapping of chromatin proteins

   Depositor

   Ulrich Laemmli

   Article

   Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

   Insert

   protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroNeo

   Plasmid

   #21740

   Depositor

   Clare Huxley

   Article

   Magin-Lachmann et al BMC Biotechnol. 2003 Feb 3. 3(1):2.

   Type

   Empty backbone

   Use

   N/a

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNHA_SBV_Gc

    Plasmid

    #208933

    Purpose

    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus Gc protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    Gc
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 1682 bp from 5' end, deleted 378 bp …

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDC32

    Plasmid

    #171204

    Purpose

    bacterial expression for HIS purification of aa1-698 of MEG-3

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    meg-3 (meg-3 Nematode)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    aa1-698

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAP20

    Plasmid

    #171206

    Purpose

    bacterial expression for HIS purification of the MEG-3 C-terminus

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    meg-3 (meg-3 Nematode)
    

    Use

    Tags

    8xHIS

    Expression

    Bacterial

    Mutation

    aa545-862

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDC25

    Plasmid

    #171203

    Purpose

    bacterial experssion of a GST-tagged MEG-3 Cterminus

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    meg-3 (meg-3 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa545-862

    Promoter

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid

    #196727

    Purpose

    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEX-A-U6-TelgRNA_PuroR

    Plasmid

    #84782

    Purpose

    Expresses telomere-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    telomere-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEX-A-U6-MaSgRNA_PuroR

    Plasmid

    #84780

    Purpose

    Expresses major satellite-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    major satellite-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEX-A-U6-MiSgRNA_PuroR

    Plasmid

    #84781

    Purpose

    Expresses minor satellite-specific sgRNA.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    minor satellite-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge