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pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Publication

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-9-10

Plasmid

#135918

Purpose

Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Publication

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-27

Plasmid

#135919

Purpose

Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Publication

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M52-303: CMV51p> Halotag-NRasHVR

Plasmid

#159686

Purpose

Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-NRasHVR (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCytERM-hfYFP

Plasmid

#186520

Purpose

Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Publication

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Expression

Mammalian

Promoter

RSV promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Jorge Ruas, Bruce Spiegelman

Publication

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Publication

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-plk1-AKAP

Plasmid

#132967

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1-AKAP (PLK1, AKAP9 Human)

Expression

Mammalian

Mutation

C terminus of full-length Plk1 fused to the peric…

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Publication

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
Anti-HCN1 [N70/28R] (Recombinant Antibody)

Antibody

#180092-rAb

Purpose

Anti-HCN1 (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended applications

Immunohistochemistry and Western Blot

Reactivity

Human, Mouse, and Rat

Source species

Mouse

Isotype

IgG2a

Trial size

Available to purchase

Available since

March 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits
R713-M48-303: CMV51p> Halotag-Kras4aHVR

Plasmid

#159684

Purpose

Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4aHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R703-M80-303: CMV51p> Halotag-Kras4bHVR

Plasmid

#159683

Purpose

Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4bHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_IB1_IIT1

Plasmid

#247080

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and T1 site. riboJ/RNaseIII site are encoded between two crRNA

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_B1_IIB5

Plasmid

#247081

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pVAM07

Plasmid

#252057

Purpose

Hygromycin B resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Publication

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGRNA1_105nt

Plasmid

#247075

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2II_LacZT1

Plasmid

#247079

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ T1 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMut19_tadDE16B

Plasmid

#247073

Purpose

L-arabinose inducible TadDE-XTEN16-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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