We narrowed to 690 results for: intein

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pET-His-SUMO-NrdJ-1N (C76A)

Plasmid

#258597

Purpose

Expression of His-SUMO tagged NrdJ-1 N intein in bacteria for protein semi-synthesis and splicing, with C76A mutation

Depositor

Thomas Muir

Article

Ye et al Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc

Insert

His-SUMO-NrdJ-1N (C76A)

Tags

5xHis-SUMO

Expression

Bacterial

Mutation

C76A mutation for avoiding disulfide bond formati…

Available Since

July 14, 2026

Availability

Academic Institutions and Nonprofits only

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pET-21_MBP-VidN

Plasmid

#241013

Purpose

VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

MBP-VidN

Tags

MBP-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

Plasmid

#162473

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

Plasmid

#162478

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreAB

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

Plasmid

#162475

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

Plasmid

#162472

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

Plasmid

#162470

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreA

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

Plasmid

#162477

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

Plasmid

#162480

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

DNE-Frterminator

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4DBD

Plasmid

#220452

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4DBD

Plasmid

#220454

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-p65AD

Plasmid

#220456

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-Gal4DBD

Plasmid

#220453

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-p65AD

Plasmid

#220455

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-p65AD

Plasmid

#220457

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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IBMc050

Plasmid

#161938

Purpose

Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI

Expression

Bacterial

Promoter

Plux2

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSABAD544

Plasmid

#45617

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_AA-GFP_C

Expression

Bacterial

Mutation

Inactivating mutations in NpuDnaE C-terminus, ste…

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His

Plasmid

#195610

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–9

Plasmid

#108187

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Insert

GFP1-9

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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