We narrowed to 1,276 results for: sumo

1. pAV106-pTDH3-mCherry-10xSumo-6xSIM

   Plasmid

   #116928

   Purpose

   Yeast integrative vector for expression of the SUMO-SIM complex

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   10xSUMO-6xSIM
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

   Plasmid

   #159107

   Purpose

   The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

   Depositor

   Thomas Tuschl

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   NSP12
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. His SUMO TEV SPOP 1-374

   Plasmid

   #197015

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

   Plasmid

   #208035

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Expression

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-30-His6-SUMO-AEY-CfaN

   Plasmid

   #122949

   Purpose

   Bacterial expression of N-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Strep Sumo

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-His6-Sumo-CfaC-MBP

   Plasmid

   #126018

   Purpose

   Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

   Depositor

   Thomas Muir

   Insert

   His6-Sumo-CfaC-MBP
   

   Use

   Tags

   6xHis-SUMO and HA-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His-SUMO- flag-hDDI2-D252N_pET11a

   Plasmid

   #169123

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D252N

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSFDuet-sumo-h-cGASCD(K427E/K428E)

    Plasmid

    #127162

    Purpose

    expression of truncated human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. H6SUMO-IntC-P53(304-393)

    Plasmid

    #200316

    Purpose

    bacterial expression of P53 TET-CTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Use

    Tags

    His6-SUMO and designed intein C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Use

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid

    #126015

    Purpose

    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-IntNtr-eSrt2A
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H14-MBP-SUMO-FLAG-LEM

    Plasmid

    #101778

    Purpose

    Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    MAN1 (LEMD3 Human)
    

    Use

    Tags

    His14-MBP-SUMO-FLAG

    Expression

    Bacterial

    Mutation

    amino acids 6-51 of human MAN1 (LEM domain)

    Promoter

    T5

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a SUMO Nup53xl 162-267

    Plasmid

    #86002

    Purpose

    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Type

    Empty backbone

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p6H-SUMO3-ORF24-NTD-Strep

    Plasmid

    #138467

    Purpose

    Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    SUMO3 and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Amino acids 2-201

    Promoter

    T7

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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