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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: sumo

Showing: 1 - 9 of 9 results

  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...site. In fact, SUMO cannot be used in eukaryotes because there is already too much SUMO protease around... its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can... solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus... your friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without...with purified protein since the enzyme cleaves the SUMO tag in the same manner as it would have in the context...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression vector for His8-SUMO tagged recombinant... pDest-Sumo - For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag Trx...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...the same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation from Cre-...S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, solubilization and purification...

  6. Distribution to Industry

    Type
    Collection
    ...types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization Christopher...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...disease Thomas Tuschl 107292 1a. pETM11-SUMO-SNCA-GFP SNCA His, SUMO T7 Parkinson's Dmytro Yushchenko 107293...pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi 133321 pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry...Nicolas Fawzi 104595 pCA528 CHMP2B 141-213 CHMP2B His, SUMO T7 ALS Wesley Sundquist 105726 pT7-7 asyn A30P SNCA... TARDBP T5 ALS Rajat Rohatgi 133322 pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi...ceroid lipofuscinosis Monther Abu-Remaileh 205054 SUMO-Twinkle 43-372 TWNK His T7 Spinocerebellar ataxia...ataxia, infantile onset Smita Patel 205055 SUMO-Twinkle 360-684 TWNK His T7 Spinocerebellar ataxia, infantile...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...

  8. Bacterial Expression Systems

    Type
    Collection
    ...Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, ...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...., Nikoniuk, A., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava...
Showing: 1 - 9 of 9 results