We narrowed to 3 results for: sumo

1. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...site. In fact, SUMO cannot be used in eukaryotes because there is already too much SUMO protease around... its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can... solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus... your friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without...with purified protein since the enzyme cleaves the SUMO tag in the same manner as it would have in the context...

2. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially...

3. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...the same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation from Cre-...S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, solubilization and purification...