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Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneUseTagsStrep-tag IIExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3 Ras-LOCKR-PL Key
Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-PL Key
Plasmid#216504PurposeRas activity dependent proximity labeler, Key only, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-PL Key
Plasmid#216505PurposeRas activity dependent proximity labeler, Key only, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-PL Key
Plasmid#216506PurposeRas activity dependent proximity labeler, Key only, ER localizedDepositorInsertER-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-PL Key
Plasmid#216507PurposeRas activity dependent proximity labeler, Key only, mitochondria localizedDepositorInsertMito-Ras-LOCKR-PL
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1-Nbox-mutant
Plasmid#194937PurposeLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL714
Plasmid#140384PurposeExpresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoterDepositorInsertT7-ctcO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL720
Plasmid#140390PurposeExpresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoterDepositorInsertT7-qacO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL722
Plasmid#140392PurposeExpresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoterDepositorInsertT7-ttgO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS mStrawberry
Plasmid#91768PurposeBacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)DepositorInsertmonomeric Strawberry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only