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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 10

Plasmid

#36925

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNGW1

Tags

NHA

Expression

Mammalian

Mutation

aa 566-1343 only

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF1a

Plasmid

#67493

Purpose

Expression of shRNA targeting human ARF1a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
HuEV-A

Plasmid

#68342

Purpose

Gateway compatible human expression vector that adds a "flexible" 3XFLAG,V5,YFP N terminus tag

Depositor

Jef Boeke

Article

Mita et al Biol Proced Online. 2016 Aug 15;18:16. d

Type

Empty backbone

Use

Cre/Lox

Tags

3xFLAG, V5 and YFP

Expression

Mammalian

Promoter

TET-CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-CACNG3

Plasmid

#140590

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CACNG3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQsupR

Plasmid

#15378

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

human H1 promoter

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-ALDH1A3

Plasmid

#140589

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA ALDH1A3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PgACR1_EYFP_pcDNA3.1

Plasmid

#161028

Purpose

Expresses PgACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

PgACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-stuffer (infusion)-IRES-CFP

Plasmid

#114727

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

stuffer (infusion)

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-SYNPO

Plasmid

#140587

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA SYNPO

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL14

Plasmid

#51794

Purpose

Inducible expression of TAL14 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL14

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Expression

Mammalian

Promoter

U6

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL21

Plasmid

#51795

Purpose

Inducible expression of TAL21 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL21

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b_RspCas13d-6xHIS

Plasmid

#251218

Purpose

Bacterial overexpression and recombinant protein purification of RspCas13d.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RspCas13d

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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