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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,229 results for: sgRNA

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Showing: 3801 - 3820 of 9229 results

  1. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  2. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  3. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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  4. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  11. pKLV2-U6gRNA5(sgBart8-5p)-PGKpuro2ABFP-W

    Plasmid
    #252919
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart8-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    252919-plasmid-map-sequence-id-508266

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  12. pKLV2-U6gRNA5(sgBart11-5p)-PGKpuro2ABFP-W

    Plasmid
    #252921
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart11-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    252921-plasmid-map-sequence-id-507824

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  16. pKLV2-U6gRNA5(sgBart10-5p)-PGKpuro2ABFP-W

    Plasmid
    #252920
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart10-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252920-plasmid-map-sequence-id-505089

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Showing: 3801 - 3820 of 9229 results