We narrowed to 7,548 results for: ski

1. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-OMP25

   Plasmid

   #141150

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. myc-KIF5B

   Plasmid

   #127617

   Purpose

   N-terminal myc-tagged mouse KIF5B

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myc-KIF5C

   Plasmid

   #127618

   Purpose

   N-terminal myc-tagged mouse KIF5C

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5C (Kif5c Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSET mNG-GECO1

   Plasmid

   #201933

   Purpose

   Expresses mNG-GECO1 indicator in bacteria

   Depositor

   Robert Campbell

   Article

   Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

   Insert

   mNeonGreenGECO1
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

   Plasmid

   #226514

   Purpose

   Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.2 YFP

    Plasmid

    #84910

    Purpose

    expression of YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

    Plasmid

    #217713

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-743

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

    Plasmid

    #217712

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FpG5

    Plasmid

    #69443

    Purpose

    lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

    Plasmid

    #217737

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR6 (ADGRG6 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-846

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

    Plasmid

    #217740

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-830

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

    Plasmid

    #217717

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2453

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

    Plasmid

    #217715

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB2 (BAI2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-917

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

    Plasmid

    #217743

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5896

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

    Plasmid

    #217742

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-412

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

    Plasmid

    #217716

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB3 (BAI3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-862

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge