We narrowed to 7,023 results for: 11

1. 3541 pMIG Bcl-XL

   Plasmid

   #8790

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bcl-XL (BCL2L1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD068

   Plasmid

   #136471

   Purpose

   SSB-GFP IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-GFP (ssb E. coli)
   

   Use

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HypoxCR

   Plasmid

   #59946

   Purpose

   A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

   Depositor

   Anne Le

   Article

   Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

   Inserts

   GFP-Pest
   mCherry-Geminin
   

   Use

   Lentiviral

   Tags

   FLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and min CMV

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFlag-tdMCP-Erk2 (pErk reporter)

   Plasmid

   #226167

   Purpose

   Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-Erk2 (MAPK1 Human)
   

   Use

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. (attB) BRaf with MS2-circRNA

   Plasmid

   #226164

   Purpose

   Used to integrate HA-tagged BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   2xHA-BRaf (BRAF Human)
   IRES-mCherry-P2A-PuroR
   MS2-circRNA barcode
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cx36-GCaMP

   Plasmid

   #123604

   Purpose

   Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

   Depositor

   John O'Brien

   Article

   Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

   Insert

   Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
   

   Use

   Tags

   GCaMP3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-RdLight1

    Plasmid

    #125708

    Purpose

    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Has Service

    AAV9

    Insert

    RdLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-RdLight1

    Plasmid

    #125707

    Purpose

    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)

    Plasmid

    #138555

    Purpose

    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2904(XI-3 MarkerFree)

    Plasmid

    #73276

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Puro-sgNT

    Plasmid

    #138681

    Purpose

    Expresses a non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2909(XII-5 MarkerFree)

    Plasmid

    #73281

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ESR1-luciferase

    Plasmid

    #113351

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ESR1-enhancer (ESR1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sc++

    Plasmid

    #155011

    Purpose

    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

    Insert

    Sc++
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1227K, Loop Substitution from S. anginosus

    Promoter

    EF1ɑ

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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