We narrowed to 9,935 results for: ADA

1. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

   Plasmid

   #200401

   Purpose

   Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Open-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis2-Arf1 (17-181)-Q71L

   Plasmid

   #184808

   Purpose

   Bacterial expression of Arf1 (aa17-181, Q71L mutation)

   Depositor

   James Hurley

   Article

   Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   aa 17-181 only, Q71L

   Promoter

   T7

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RalGDS-YPet

   Plasmid

   #134929

   Purpose

   Fluorescently tagged affinity reagent

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   RaIGDS RBD, YPet
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Orange Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71346

   Purpose

   Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

5. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

   Plasmid

   #191145

   Purpose

   Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   3UTR(Bdnf)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_nLightR

   Plasmid

   #217657

   Purpose

   Expresses norepinephrine indicator nLightR under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSIIbsr-6013x

   Plasmid

   #108654

   Purpose

   An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   Raichu-EV
   

   Use

   Lentiviral

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACT2-μ3A

   Plasmid

   #198174

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitution in codon 225

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACT2-μ3B

   Plasmid

   #198175

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3B
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

    Plasmid

    #86871

    Purpose

    To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    SF3B6 (SF3B6 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    amino acids 7-29 deletion

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-HA C/EBP gamma

    Plasmid

    #15715

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    C/EBP gamma (CEBPG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAV1-K*R-HA

    Plasmid

    #27765

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

    Plasmid

    #191141

    Purpose

    Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-W:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    UAG>UGG (stop>Trp)

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRP6-m5-pCS2-VSVG

    Plasmid

    #27283

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    Changed S/T of all five PPP(S/T)P motifs to Alani…

    Promoter

    Available since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGE006

    Plasmid

    #108722

    Purpose

    Cas9 expression in M.polymorpha

    Depositor

    Shigeo Sugano, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Insert

    Atco-Cas9-Pea3ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEFpro

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC_N_LHX1

    Plasmid

    #167775

    Purpose

    Expresses LHX1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    LHX1 (LHX1 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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