We narrowed to 4,193 results for: Pet2

1. pKD077

   Plasmid

   #136473

   Purpose

   SSB-mTur2 IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-mTur2 (ssb E. coli)
   

   Use

   Tags

   mTurquoise2

   Expression

   Bacterial

   Mutation

   mTurquoise2 inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHYP-NPC-10H

   Plasmid

   #162789

   Purpose

   SARS-CoV-2 Nucleocapsid Protein expression in E.coli

   Depositor

   Ivan Vorobiev

   Article

   Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

   Insert

   Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a+GFP-CAMSAP3 CKK

   Plasmid

   #59033

   Purpose

   expresses CKK domain of CAMSAP3 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CKK domain (CAMSAP3 Mouse)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETNKI-his-SUMO-RNF168ring (1-189)

   Plasmid

   #63570

   Purpose

   Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   6xHis and SUMO3

   Expression

   Bacterial

   Mutation

   Ring domain only (residues 1-189)

   Promoter

   T7

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSunS

   Plasmid

   #171672

   Purpose

   SunS expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFP-FRBx5-His

   Plasmid

   #103784

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   CFP-FRBx5-His
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-FKBPx5-His

   Plasmid

   #103785

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-FKBPx5-His
   

   Use

   Tags

   EYFP, His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pZ005 (AacC2c1 human codon optimized protein)

    Plasmid

    #70171

    Purpose

    Expresses AacC2c1 human codon optimized protein

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 protein
    

    Use

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a+GFP-CAMSAP1 CKK

    Plasmid

    #59031

    Purpose

    expresses CKK domain of CAMSAP1 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 CKK domain (CAMSAP1 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMtb CblA

    Plasmid

    #138298

    Purpose

    Expression of Mtb CblA

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Mtb CblA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMtb ATR

    Plasmid

    #138297

    Purpose

    Expression of Mtb ATR

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Insert

    Corrinoid adenosyl transferase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNIC28-Bsa4-ICL1

    Plasmid

    #105822

    Purpose

    Expression of Mycobacterium tuberculosis icl1 recombinant protein

    Depositor

    Ivanhoe Leung

    Article

    Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

    Insert

    icl1
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHis-hPim1

    Plasmid

    #100722

    Purpose

    Expression of human Pim1 kinase domain in E.coli

    Depositor

    Byung Il Lee

    Article

    Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

    Insert

    Pim1 (PIM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 29-313 of isoform 2

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GRLF1

    Plasmid

    #36195

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains the Ras homolog domain only (amino acid …

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. HpMscS

    Plasmid

    #78310

    Purpose

    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

    Depositor

    Douglas Rees

    Article

    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

    Insert

    Mechanosensitive channel of small conductance
    

    Use

    Tags

    6x His Tag and FLAG Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. NmMetQ N238A

    Plasmid

    #129648

    Purpose

    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

    Depositor

    Douglas Rees

    Article

    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

    Insert

    metQ Gene
    

    Use

    Tags

    10x Histidine Tag, Enterokinase, and pelB signal …

    Expression

    Bacterial

    Mutation

    Changed Asparagine 238 to Alanine

    Promoter

    T7 Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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