We narrowed to 23,834 results for: Spr;

1. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149427

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[TraB, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVMG0193_Cq-Rpl40-Cas9

   Plasmid

   #169346

   Purpose

   Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0193_Cq-Rpl40-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CPIJ002413

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-sgCTRL-1

   Plasmid

   #83934

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgCTRL-2

   Plasmid

   #83935

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-sgCTRL-3

   Plasmid

   #83936

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPGK-ABE7.10-P2A-GFP

   Plasmid

   #162511

   Purpose

   Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

   Insert

   ABE7.10-P2A-GFP
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EFS-ABE7.10-P2A-GFP

   Plasmid

   #162510

   Purpose

   Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

   Insert

   ABE7.10-P2A-GFP
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-u(XXL)Cas9

   Plasmid

   #127387

   Purpose

   Expresses Cas9 at very low level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(XXL)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-FnCas12a-TLR-MCV1-gRNA

   Plasmid

   #117412

   Purpose

   FnCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   FnCas12a gRNA targeting TLR 2.0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AcrIIC1-mCherry chimera #10

    Plasmid

    #128113

    Purpose

    Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrIIC1-mCherry chimera #10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9FnCpf1GG

    Plasmid

    #131013

    Purpose

    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

    Plasmid

    #105021

    Purpose

    Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Zfp219 (Zfp219 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

    Plasmid

    #105030

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

    Plasmid

    #105031

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W

    Plasmid

    #105033

    Purpose

    Lentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Vps25 (Vps25 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

    Plasmid

    #105016

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tcf7l1 (Tcf7l1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLK4 G8.1 gRNA

    Plasmid

    #90837

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK4 (Guide Designation G8.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PLK4 G7.1 gRNA

    Plasmid

    #90836

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK4 (Guide Designation G7.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MIS18BP1 F10.1 gRNA

    Plasmid

    #90769

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MIS18BP1 (Guide Designation F10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MCM6 E1.2 gRNA

    Plasmid

    #90762

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MCM6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MCM6 (Guide Designation E1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge