We narrowed to 11,754 results for: pcDNA

1. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Flag-mRIPK1-S321E

   Plasmid

   #115340

   Purpose

   Mammalian expression of Flag-mRIPK1-S321E

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321E

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)Flag-His-ATM wt

   Plasmid

   #31985

   Purpose

   Mammalian expression of human ATM with Flag and His tags

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM wild-type (ATM Human)
   

   Use

   Tags

   Flag and HIS

   Expression

   Mammalian

   Mutation

   3' UTR of 400 nucl.

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA GFP10-ZIP-GFP11

   Plasmid

   #182243

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP-11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA4/Full length FVIII

   Plasmid

   #41036

   Depositor

   Robert Peters

   Article

   Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

   Insert

   FVIII (F8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-PdCO-mScarlet

   Plasmid

   #198507

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-10His-mStayGold (E138D)

   Plasmid

   #211363

   Purpose

   Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   E138D

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Use

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO HIS HSPA8

    Plasmid

    #19541

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO GFP HSPH1

    Plasmid

    #19480

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EGFP-Cdc42(Q61L)

    Plasmid

    #12600

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (Q61L) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid

    #66931

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 LIC 6A CARD8-FLAG

    Plasmid

    #169948

    Purpose

    Mammalian expression of CARD8-FLAG

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT/TO Plk4-mCherry

    Plasmid

    #80269

    Purpose

    mammalian expression plasmid for Plk4-mCherry

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IRE1 alpha KA-pcDNA3.EGFP

    Plasmid

    #13010

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R4L, K599A

    Promoter

    CMV

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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