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Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform-2)

Plasmid

#245901

Purpose

Mammalian expression of CDKL5 isoform2 (1-1030) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-1 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CDKL5(kinase)

Plasmid

#245902

Purpose

Mammalian expression of CDKL5 kinase domain (1-311) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CDKL5(isoform1)-GFP

Plasmid

#245903

Purpose

Mammalian expression of CDKL5 isoform1 (1-960) with C-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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M79

Plasmid

#242631

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYCdeltaCTD (MYC Human)

Use

RNAi

Tags

V5

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M91

Plasmid

#242635

Purpose

lower MYCdeltaCTD expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYCdeltaCTD (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M88

Plasmid

#242634

Purpose

MYCdeltaCTD expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

EGFP-MYCdeltaCTD (MYC Human)

Expression

Mammalian

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M93

Plasmid

#242637

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYC (MYC Human)

Use

RNAi

Tags

V5

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M99

Plasmid

#242638

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M92

Plasmid

#242636

Purpose

lower MYC expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYC (MYC Human)

Tags

V5

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M76B

Plasmid

#242628

Purpose

MYCMutMBIV expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIV (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M77B

Plasmid

#242630

Purpose

MYCMutMBIIIa expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M76

Plasmid

#242627

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIV (MYC Human)

Use

RNAi

Tags

V5

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-DNAJB1n_CAGEn (Nrdj1)

Plasmid

#241422

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product DNAJ-PKAc, Figure 4, Extended Figure 8-9 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

DNAJB1n_CAGEn (Nrdj1) (DNAJB1 Human, Synthetic)

Tags

Myc

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1394

Plasmid

#143104

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF569 (ZNF569 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

Plasmid

#222287

Purpose

Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

Depositor

Anne Straube

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

HOOK3 (HOOK3 Human)

Tags

8 His and ZZ tag

Expression

Insect

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Rat, Synthetic)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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