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Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgNT
Plasmid#140238PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNADepositorInsertnon-targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_HMOX1-V5-APEX2
Plasmid#85823PurposeExpressed C-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertHMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
UseTagsExpressionMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(0)
Plasmid#62891PurposeTrojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M
Plasmid#140315PurposeExpresses Flag-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
myristoylated ct-GRK2
Plasmid#178734PurposeN-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)DepositorInsertGRK2 (GRK2 Bovine)
UseLentiviralTagsSrc 1-15ExpressionMutationonly amino acids 548-671PromoterUbiquitinAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAD-KPS12-Pman
Plasmid#133832PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LENC
Plasmid#111163PurposemiR-E (miR-30 variant)-based RNAi with mCherry reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-5102HRasCT(Picchu)
Plasmid#209917PurposeA FRET biosensor for tyrosine kinase activityDepositorInsert5102HRasCT
UseTagsYPet and nTurquoise-GLExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro
Plasmid#211687PurposeU6-sgRNA-EFS-Cas9-P2A-PuroDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2Δ751-813aa
Plasmid#163603PurposeExpresses BMPR-2 lacking the LIMK-binding region in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe LIMK-binding region (751-813 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PXL
Plasmid#75349PurposePX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.DepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SGEN
Plasmid#111171PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterSFFVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only