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We narrowed to 965 results for: REP

Showing: 1 - 20 of 965 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from ...on each item's material page. Actual titers are reported with each shipment. For more details about our...of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...

  2. AAV Viral Preps

    Type
    Collection
    ... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...page. Actual titers are reported with each shipment. If you can't find the prep you need, try Addgene's...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...service. You can choose a transfer plasmid from our repository and request packaging in one of seven serotypes...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster... pages above. You can also do a search of viral preps and filter by the serotype needed....

  3. Biosensor AAV Preps

    Type
    Collection
    ...iGluSnFr SF-iGluSnFr Glucose Sensors iGlucoSnFr Norepinephrine (NE) Sensors GRAB_NE nLight Oxytocin (OT) Sensors...iGlucoSnFr mRuby2 Constitutive 9 Loren Looger Norepinephrine (NE) Sensors 123308 pAAV-hSyn-GRAB_NE1m Syn...control) none Constitutive 1, 9 Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...-ST none Cre dependent 1 GENIE Project Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5-Kv2.1-WPRE Syn ASAP5...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...Bryan Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent...Ulrik Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  5. Control AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  7. Recombinases AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Yulong Li 123309 pAAV-hSyn-GRAB_NE1h...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Yulong Li 123310 pAAV-hSyn-GRAB_NEmut... GRAB-NEmut control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Yulong Li 20297...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...GCaMP6f GCaMP6s GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors...

  9. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional repression of target genes.... (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

  10. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Collections Bikard Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like...CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with...the guide RNA and the target sequence lower the repression level. By controlling the number of mismatches...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...the phage 186. Detailed information about the repression system can be found in this article: Vigouroux...10.15252/msb.20177899. PMID: 29519933 . bioRxiv preprint . Detailed information about chromosomal integration...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes ...packaging plasmids (Rep/Cap plasmids) in Addgene’s repository that express AAV Rep and Cap genes for packaging...Service This table lists the packaging plasmids (Rep/Cap plasmids) used by Addgene's Viral Vector Packaging...Plasmids Packaging plasmids, or RepCap plasmids, encode the AAV replication and capsid proteins necessary...expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2...expressing Rep2 and Cap7 James M. Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2 and Cap8...expressing Rep2 and Cap9 James M. Wilson 240486 pAAV2/11 v1 AAV11 AAV packaging plasmid, expressing Rep2 and ...

  12. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options include...and comprehensive quality control. In-Stock Preps 1,000+ preps ready to ship immediately after MTA approval...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...options so you can easily access the viral vector prep you need to get your experiments started more quickly...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. ...Mouse Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells. Cell Rep. 2014 Dec 11...Transcription Factor Induction Mediates Hemogenic Reprogramming. Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. Pereira...inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. Kay and Wu MMLV-derived Retrovirus...Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts ...pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome...variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. ...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...visualization of Ca 2+ dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red...genetically encoded fluorescent indicators. Cell Rep Methods. 2023 Jul 27;3(8):100544. Peng Zou Calcium...microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent...applicable to optogenetics and in vivo imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic...Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. Xavier Nicol cyclic...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose ...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Pyruvate...

  15. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Other citations ...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Rosario, et al. ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...Other citations include: Chen, et al. 2014. Reprogramming adipose tissue-derived mesenchymal stem cells...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...contact with other organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions...on the NeuroMabSeq platform. (Mitchell et al. Sci Rep. 2023) See More Fluorescent Proteins Find information... al. Neuron. 2019) See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral particles... Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent...swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT)...(Ehrlich et al. Proc Natl Acad Sci USA. 2017) Reprogram astrocytes to dopaminergic neurons using three...

  17. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913...

  18. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...Minisymposium on Reproducibility. The minisymposium focused on solutions to reproducibility issues in the...own talk on reproducibility at Addgene and here I summarize what I learned both in preparation for my talk...ways of defining scientific reproducibility. For a study to be “reproducible” does not necessarily mean...someone has replicated it precisely. You can find a great discussion of different types of replication in Nosek...concepts. However, rates of reproducibility for preclinical studies, as reported by drug companies attempting... the Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed... enterprise towards greater reproducibility. Cause 1: Inability to repeat previously performed experiments...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...site-directed mutagenesis by recombineering.” Sci Rep 6:19121. PubMed PMID: 26750263. PubMed Central PMCID: PMC4707547...cost. Read on to find out how to replace your SDM method with REPLACR (Recombineering of Ends of Linearized...al., REPLACR-mutagenesis’ median efficiency is 75%. If these numbers hold up across labs, REPLACR-mutagenesis... time. Let us know how REPLACR-mutagenesis works in your hands!     REPLACR-Mutagenesis Megaprimer ...costly in vitro assembly methods. A new method, REPLACR-mutagenesis, harnesses the power of bacterial recombineering...are then transformed into E. coli to undergo nick repair. This method can work well, but poor template digestion...secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria do the work If you...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian...antigen binding sequence to improve binding and reproducibility. Some scientists choose to work with full length...
Showing: 1 - 20 of 965 results