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We narrowed to 117 results for: REP

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Showing: 1 - 20 of 117 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...reduce binding with Rep proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important...persistence. They work in conjunction with the viral rep (replication) and cap (capsid) proteins, which bind...). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding site...resolution site that serves as the target site for Rep proteins (Savvy et al. 2013). Figure 2: AAV2 wild...-C’ regions) and forms a T-shaped structure. The Rep binding element (RBE) and terminal resolution site...productivity but increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...Created with BioRender.com   Replication (rep) gene The rep gene encodes four proteins that are required...Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins...domains, all four Rep proteins share a common helicase and ATPase domains. To function, the Rep proteins form...RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV... when recombinant AAV (rAAV) is made in the lab, rep and cap are expressed from a separate plasmid in ...virus instead.  Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid...replication and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene expression is often driven by the AAV p5 or ...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...interconvertible Drosophila overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...entry The viral packaging proteins for AAV are rep and cap. Rep is required for viral replication and also...release from late endosomes when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015 Nov 5;5:16277. PubMed PMID: 26538064. PubMed...-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016 Mar 4;6:22555. PubMed PMID: 26939770. PubMed...-1 Replication and Accelerate Viral Escape. Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan V, Shlomai...DNA efficiently suppresses hepatitis B virus. Sci Rep. 2015 Jun 2;5:10833. PubMed PMID: 26035283. PubMed...

  9. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP...photoconversion of GFP for protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed...

  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes ...packaging plasmids (Rep/Cap plasmids) in Addgene’s repository that express AAV Rep and Cap genes for packaging...Service This table lists the packaging plasmids (Rep/Cap plasmids) used by Addgene's Viral Vector Packaging...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. Ptashne Minicircle ...inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. Kay and Wu MMLV-derived Retrovirus...fibroblasts to hematopoietic progenitor cells. Cell Rep. 2014 Dec 11;9(5):1871-84. Lacaud Fibroblasts Hematopoietic...Induction Mediates Hemogenic Reprogramming. Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. Pereira Fibroblasts...Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...visualization of Ca 2+ dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red...genetically encoded fluorescent indicators. Cell Rep Methods. 2023 Jul 27;3(8):100544. Peng Zou Calcium...microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent...applicable to optogenetics and in vivo imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic...Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. Xavier Nicol cyclic...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose ...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Pyruvate...

  18. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-018-33408-9 Mizuno...Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038/srep32565 Resources ...
Showing: 1 - 20 of 117 results