...in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from ...on each item's material page. Actual titers are reported with each shipment. For more details about our... that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific...of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...

... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...page. Actual titers are reported with each shipment. If you can't find the prep you need, try Addgene's...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...service. You can choose a transfer plasmid from our repository and request packaging in one of five serotypes...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster... pages above. You can also do a search of viral preps and filter by the serotype needed....

...iGluSnFr SF-iGluSnFr Glucose Sensors iGlucoSnFr Norepinephrine (NE) Sensors GRAB_NE nLight Oxytocin (OT) Sensors...cyto)iGlucoSnFr mRuby2 Constitutive 9 Looger Norepinephrine (NE) Sensors 123308 pAAV-hSyn-GRAB_NE1m Syn...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent...* Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Li 123309 pAAV-hSyn-GRAB_NE1h...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Li 123310 pAAV-hSyn-GRAB_NEmut... GRAB-NEmut control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Li 20297 pAAV-EF1a-double...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...GCaMP6f GCaMP6s GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors...

...CRISPR plasmids for transcriptional repression of target genes....Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information ...

...Collections Bikard Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like...CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with...the guide RNA and the target sequence lower the repression level. By controlling the number of mismatches...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...the phage 186. Detailed information about the repression system can be found in this article: Vigouroux...10.15252/msb.20177899. PMID: 29519933 . bioRxiv preprint . Detailed information about chromosomal integration...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...

... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat Plasmids...single TALE repeat domains. TALengineering...rapid and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as ... set of 28 plasmids each encoding a single TALE repeat domain and flanked by restriction sites used for...

...Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options include...started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...Service Addgene produces high-quality viral vector preps from select plasmids in our collection. We know ...high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo...high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse...Request Can't find what you need in our in-stock preps? Our Packaged on Request service offers you even...

...motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce...system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural oxytocin receptors using...cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...on the NeuroMabSeq platform. Mitchell et al. Sci Rep. 2023 Sep 27. See More Fluorescent Proteins Find ...transitions in protein self-organization. Wan et al. Cell Rep Methods. 2022 Mar 28. Follow APP processing and dynamics...Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral particles... Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent...

...Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. ...Mouse Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells. Cell Rep. 2014 Dec 11...Transcription Factor Induction Mediates Hemogenic Reprogramming. Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. Pereira...inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. Kay and Wu MMLV-derived Retrovirus...Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts ...pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome...variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. ...

...visualization of Ca(2+) dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red...microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent...applicable to optogenetics and in vivo imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic...Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. Xavier Nicol cyclic...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose ...for biochemical assays and live cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Sucrose ...pH measurements in the endoplasmic reticulum. Sci Rep. 2018 Aug 10;8(1):11985. Eckhard Boles pH Imaging...

...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Other citations ...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Rosario, et al. ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...Other citations include: Chen, et al. 2014. Reprogramming adipose tissue-derived mesenchymal stem cells...

...characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913...

...cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian...antigen binding sequence to improve binding and reproducibility. Some scientists choose to work with full length...

...encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding AAV Rep and respective capsid protein which...expressing Rep2 and Cap1 James M. Wilson 104963 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2...expressing Rep2 and Cap5 Melina Fan 112863 pAAV2/7 AAV7 AAV packaging plasmid, expressing Rep2 and Cap7 ...expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging plasmid, expressing Rep2 and Cap9...advantage of using AAV for research is that it is replication-limited and typically not known to cause disease...certain exogenous gene products that mediate AAV replication. This requirement has been circumvented with ...learn about our options for hundreds of in-stock preps that are ready to ship right away, as well as our...

...performed using the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding...order to generate high quality preparations. After production, all virus preps are titered and subjected to... containing) AAV particles within representative vector preparations was quantified with electron microscopy...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation...purity and titers adequate for in vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer...on viral genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the...