We narrowed to 63,106 results for: REP
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Plasmid Kit#1000000213PurposeA set of plasmids containing barcoded self-reporting transposons used for 'calling card' experiments to identify transcription factor binding sites and changes in gene expression.DepositorAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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pMC-6-BeYDV-Rep-RepA-9
Plasmid#197715PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV Rep/RepA proteins: native ORFs
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-BeYDV-Rep-RepA-9
Plasmid#197715PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV Rep/RepA proteins: native ORFs
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralPromoterCMVAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)
Plasmid#225432PurposeTranscriptional unit of BeYDV Rep/RepA (C1:C2)DepositorInsertpNOS:Rep/RepA:tNOS
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralPromoterCMVAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)
Plasmid#225432PurposeTranscriptional unit of BeYDV Rep/RepA (C1:C2)DepositorInsertpNOS:Rep/RepA:tNOS
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)
Plasmid#225433PurposeTranscriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.DepositorInsert4xCBS:mDFR:Rep/RepA:Tnos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)
Plasmid#225433PurposeTranscriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.DepositorInsert4xCBS:mDFR:Rep/RepA:Tnos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
ExpressionMammalianPromoterU6Available SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
ExpressionMammalianPromoterU6Available SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only