Adeno-associated virus (AAV) Guide
Type
Guide
...reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the...to specific Rep-binding elements present in the AAV ITRs and the AAVS1 site. Since the Rep region is removed...Repeat, contains a Rep binding element (RBE) that serves as a binding site for Rep proteins essential ...required but often included. Packaging plasmid Rep in trans The rep gene encodes four proteins that are required...Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the two ITRs with a gene ...