Addgene

We narrowed to 4,639 results for: Drosophila

Showing: 3861 - 3880 of 4639 results

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pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

Plasmid

#62944

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-C3-PA

Plasmid

#175446

Purpose

HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

3C-Protein A and BiP Secretion Peptide

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-Fc-His

Plasmid

#72190

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET11-PC-H6

Plasmid

#183777

Purpose

Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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cmv hTid Long

Plasmid

#13707

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long (DNAJA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJC-ase0.8kb-TALE3-VP64-P10

Plasmid

#104610

Purpose

Expresses TALE3 under the control of a 0.8kb ase enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

0.8kb ase enhancer element (ase Fly)

Use

TALEN

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUASt-SIK3 S563A

Plasmid

#52976

Purpose

insect expression of SIK3 S563A PKA phosphorylation defective mutant

Depositor

Biao Wang, Marc Montminy

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Use

Tags

HA

Expression

Insect

Mutation

S563A PKA phosphorylation defective

Promoter

Hsp70

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pUASp-Flag-Myc-Qin-E3-attB

Plasmid

#44209

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin (qin Fly)

Use

Fly transformation

Tags

3xFlag and 6xMyc

Expression

Mutation

lacking 5xTudor domains on C-term; aa1-274 only

Promoter

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Use

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUASt-Xbp1-HA-GFP-3'UTR Spl

Plasmid

#201246

Purpose

Xbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced form

Depositor

Pedro Domingos

Article

Cairrao et al Nat Commun. 2022 Mar 24;13(1):1587. doi:

Insert

dXbp1 (Xbp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz_1213-2103_AH

Plasmid

#148887

Purpose

Insect Expression of DmHelz_1213-2103

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1213-2103 (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz-E958Q_AH

Plasmid

#148888

Purpose

Insect Expression of DmHelzE958Q

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelzE958Q (CG9425 Fly)

Use

Tags

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot3_1-212_AF

Plasmid

#148737

Purpose

Bacterial Expression of HsNot3_1-212

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot3_1-212 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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