We narrowed to 24,898 results for: Spr

1. pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179053

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SPC42-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

   Plasmid

   #179033

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Neon(gfp)-Link-TUB1
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PPP1CB C3.5 gRNA

   Plasmid

   #90845

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PPP1CB

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PPP1CB (Guide Designation C3.5)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458_BCL6_iso1_2

   Plasmid

   #104047

   Purpose

   Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   BCL6_iso1 gRNA (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_BCL6_iso1_1

   Plasmid

   #104046

   Purpose

   Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   BCL6_iso1 gRNA (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ANLN A1.2 gRNA

   Plasmid

   #90530

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ANLN

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ANLN (Guide Designation A1.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTox-EGFPsite1_NGA-PAM (BPK754)

   Plasmid

   #181747

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
   

   Use

   Selection plasmid

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AURKA A2.1 gRNA

   Plasmid

   #90541

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AURKA

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   AURKA (Guide Designation A2.1)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIP13 H3.2 gRNA

    Plasmid

    #90920

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIP13

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TRIP13 (Guide Designation H3.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AcrIIC1X

    Plasmid

    #128112

    Purpose

    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX (AcrIIC1 N3F/D15Q/A48I)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GMNN D1.2 gRNA

    Plasmid

    #90696

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GMNN

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    GMNN (Guide Designation D1.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. UBA1 G5.3 gRNA

    Plasmid

    #90932

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human UBA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    UBA1 (Guide Designation G5.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_CEBPG_1

    Plasmid

    #86344

    Purpose

    Encodes gRNA for 3' target of human CEBPG

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against CEBPG (CEBPG Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_CEBPA_2

    Plasmid

    #86359

    Purpose

    Encodes gRNA for 3' target of human CEBPA

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against CEBPA (CEBPA Human)
    

    Use

    CRISPR

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-gRNA-TAZ

    Plasmid

    #86688

    Purpose

    Expresses a gRNA targeted to human TAZ

    Depositor

    William Pu

    Article

    Wang et al Nat Protoc. 2017 Jan;12(1):88-103. doi:

    Insert

    U6-gRNA-TAZ (TAFAZZIN Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEH2A B9.3 gRNA

    Plasmid

    #90878

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RNASEH2A (Guide Designation B9.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Bba_R0040 TetR

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLTC G10.4 gRNA

    Plasmid

    #90635

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CLTC

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CLTC (Guide Designation G10.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY036_ATP1A1_G5

    Plasmid

    #86618

    Purpose

    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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