We narrowed to 15,667 results for: nar

1. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

   Plasmid

   #120302

   Purpose

   AAV vector for expression of AcrIIC1 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

   Plasmid

   #120303

   Purpose

   AAV vector for expression of AcrIIC3 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC3-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CRY-GalVP16 (B695)

    Plasmid

    #92031

    Purpose

    Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    GalBD-VP16AD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. nanos-PCRII

    Plasmid

    #26601

    Depositor

    Phillip Newmark

    Article

    Wang et al Proc Natl Acad Sci U S A. 2007 Apr 3. 10

    Insert

    nanos
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBS-germinal histone H4

    Plasmid

    #26600

    Depositor

    Phillip Newmark

    Article

    Wang et al Proc Natl Acad Sci U S A. 2007 Apr 3. 10

    Insert

    germinal histone H4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. PL05016A1H10-ilp-1

    Plasmid

    #26522

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    ilp-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pJC109.1-spp-12

    Plasmid

    #26513

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    spp-12
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pJC130.1-npp-18

    Plasmid

    #26505

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    npp-18
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pJC162.1_pc2RNAi

    Plasmid

    #26541

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    prohormone convertase 2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pJC100.1-ppl-1

    Plasmid

    #26519

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    ppl-1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pJC114.4-spp-2

    Plasmid

    #26509

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    spp-2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pBL4.8-eye53-1

    Plasmid

    #26533

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    eye53-1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only