We narrowed to 4,064 results for: gast

1. pAc5.1B-lambdaN-HA-DmTral-S13AI15A_C

   Plasmid

   #146081

   Purpose

   Insect Expression of DmTral-S13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-S13AI15A (tral Fly)
   

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

   Plasmid

   #146071

   Purpose

   Insect Expression of DmTral-LSmR21EE23KR42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KR42EF44A (tral Fly)
   

   Expression

   Insect

   Mutation

   R21E, E23K, R42E, F44A. Additionally, one silent …

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmTral-R42EF44A_C

   Plasmid

   #146061

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Expression

   Insect

   Mutation

   R42E, F44A. Additionally, two silent mutations A1…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

   Plasmid

   #112987

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Mutation

   S123-125-3A

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

    Plasmid

    #112985

    Purpose

    In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Mutation

    S123-125-3D

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

13. KHBD00320

    Plasmid

    #34445

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6376 (E2f1 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pMXs-IP-mCherry-TOSI

    Plasmid

    #172496

    Purpose

    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    PDCD4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    Mcherry

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2 puro - CRBN (#1)

    Plasmid

    #166240

    Purpose

    sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR v2 puro - CRBN (#2)

    Plasmid

    #166241

    Purpose

    sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pUCIDT-attL1-Human ABeta-attR5

    Plasmid

    #160436

    Purpose

    Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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