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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3901 - 3920 of 18824 results

  16. PHGDH

    Plasmid
    #38841
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHGDH (PHGDH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. deltaVP1 + VP2C-GFPdeg

    Plasmid
    #166677
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166677-plasmid-map-sequence-id-329832

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  18. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Publication
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

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  19. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid
    #167943
    Purpose
    SFTPC reporter targeting repair template
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SFTPC (SFTPC Human)
    Use
    CRISPR; Donor template
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    EF1a
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167943-plasmid-map-sequence-id-331139

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  20. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid
    #104569
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x2)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104569-plasmid-map-sequence-id-201433

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Showing: 3901 - 3920 of 18824 results