We narrowed to 8,244 results for: pCMV

1. pCMV5 mouse src K295R Y527F

   Plasmid

   #13657

   Depositor

   Peter Howley, Joan Brugge

   Insert

   src K295R Y527F (Src Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K295R Y527F. Dominant negative mutant.

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. p155 pCMV-EGFP/beta-gal

   Plasmid

   #8387

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox; Beta-gal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

   Plasmid

   #163554

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV:: NES-FKBP-iLID-LRP6c

   Plasmid

   #221774

   Purpose

   Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

   Insert

   pCMV:: NES-FKBP-iLID-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SlugCBE-NNG-P2A-puro

   Plasmid

   #214355

   Purpose

   Expresses SlugCBE-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCBE-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFlag pCMV5-TOPO TAZ (S89A)

   Plasmid

   #24815

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Serine 89 mutated to Alanine

   Promoter

   Available since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV-FLEx-TetOn3G-3xHA-FLEx

   Plasmid

   #133729

   Purpose

   Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G-3xHA (floxed & inverted)
   

   Use

   AAV

   Tags

   3xHA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

   Plasmid

   #169586

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

    Plasmid

    #74262

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-E1347A-nCas9(D10A)-UGI

    Plasmid

    #187410

    Purpose

    DddA E1347A variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA E1347A variant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCMV-intron myc Rab6 T27N

    Plasmid

    #46782

    Purpose

    expression of T27N Rab6a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab6 T27N (RAB6A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T27N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid

    #58370

    Purpose

    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    SNAP and vhhGFP4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-AAAAA-nCas9(D10A)-UGI

    Plasmid

    #187409

    Purpose

    DddA AAAAA variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA AAAAA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-TM-CIBN-BLITz1-TetR-VP16

    Plasmid

    #89878

    Purpose

    expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    TM-CIBN-TevN-BLITz1-TetR-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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