We narrowed to 7,284 results for: Ank

1. INCTbiosyn-pEF-GFP(rc)2

   Plasmid

   #127504

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST CDC42EP1 WT

   Plasmid

   #69819

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUBP1

   Plasmid

   #155613

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   FUBP1 (FUBP1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-mCherry-2A-BRD4 Iso A

   Plasmid

   #137720

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn2delBRCT

   Plasmid

   #173939

   Purpose

   Bacterial expression of PARP1 lacking Zn2 and BRCT domains

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 and 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1deltaART

   Plasmid

   #174804

   Purpose

   Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1deltaART (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 785-1014

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Trim46

   Plasmid

   #176402

   Purpose

   TRIM46 tagged with GFP

   Depositor

   Casper Hoogenraad, Thomas Schwarz

   Article

   van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

   Insert

   trim46 (Trim46 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1408

    Plasmid

    #29205

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-Trim46

    Plasmid

    #176401

    Purpose

    TRIM46 tagged with mCherry

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-sgAAVS1

    Plasmid

    #85802

    Purpose

    Cas9/CRISPR vector for cut at human AAVS1 gene intron1

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Expression

    Mammalian

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57-mCherry-2A-BRD4 Iso C

    Plasmid

    #137721

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 short isoform (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX301-ARF6-TagRFP

    Plasmid

    #162029

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CPSF4

    Plasmid

    #155477

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CPSF4-1

    Plasmid

    #155478

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge