We narrowed to 166,040 results for: addgene

1. pAV-U6+27-tCorn

   Plasmid

   #106233

   Purpose

   Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

   Insert

   tRNA-Corn
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK DN Rac1

   Plasmid

   #84642

   Purpose

   Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

3. MCP-ADAR2-DD(E488Q)

   Plasmid

   #170125

   Purpose

   Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MCP-ADAR2-DD(E488Q)-NES
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1a core promoter

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiGuide_GFP_NT135

   Plasmid

   #183118

   Purpose

   pLentiGuide modified to express GFP and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. P1-EYFP-pA (Construct 5)

   Plasmid

   #55197

   Purpose

   Synthetic promoter responsive to gRNA1

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Minimal Ade MLP

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-GFP-CtIP

   Plasmid

   #71109

   Purpose

   mammalian expession of GFP-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIK CA Rac1

   Plasmid

   #84605

   Purpose

   Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Constitutively Active, Q61L

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pSLIK DN RhoA

   Plasmid

   #84646

   Purpose

   Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   RhoA (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pMH-HA/Joseph2-Multitagged Plasmid

    Plasmid

    #226726

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pMH-HA/Joseph2
    

    Tags

    HA, Myc, GFP, Flag, V5, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK_hygro_BirA

    Plasmid

    #136537

    Purpose

    Lentiviral expression vector for an inducible BirA

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Expression

    Mammalian

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. P2-ECFP-pA (Construct 6)

    Plasmid

    #55198

    Purpose

    Synthetic promoter responsive to gRNA2

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    Minimal Ade MLP

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. HLA_DRA_01_01

    Plasmid

    #165012

    Purpose

    Expression of HLA-DRA*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRA (HLA-DRA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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