We narrowed to 59,432 results for: cloning

1. pDONR223 MERS-CoV NSP6_stop

   Plasmid

   #168633

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   NSP6 (orf1ab )
   

   Use

   Other

   Available Since

   July 5, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDONR223 MERS-CoV NSP3_nostop

   Plasmid

   #168608

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   NSP3 (orf1ab )
   

   Use

   Other

   Available Since

   Jan. 20, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSCBE3-NG

   Plasmid

   #218159

   Purpose

   This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / L1111…

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-Mef2c-tPT2A-GFP

   Plasmid

   #111771

   Purpose

   Bicistronic construct: Co-express two genes by 2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Insert

   Mef2c-tPT2A-GFP (Mef2c Mouse)
   

   Use

   Cloning intermediate

   Tags

   GFP

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-Trastuzumab-IgA2/κ

   Plasmid

   #61889

   Purpose

   Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
   HER2/neu receptor specific humanized kappa light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDFNKI-strepII-3C-LIC-strep

   Plasmid

   #108708

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8/GW/TOPO-TBX20a

   Plasmid

   #98620

   Purpose

   Gateway entry TOPO TA cloning vector for TBX20a

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Tbx20a (Tbx20 Mouse)
   

   Use

   Topo ta cloning vector

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221-EF1.Pro

   Plasmid

   #79488

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   EF1 Promoter (EEF1A1 Synthetic, Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   EF1 Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETNKI-strepII-3C-LIC-Amp

   Plasmid

   #108707

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-IL6.Pro

    Plasmid

    #79491

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Il6 Promoter (IL6 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    IL6 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid

    #100716

    Purpose

    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR4-Cas9-V5

    Plasmid

    #87905

    Purpose

    Cloning vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    Cas9-V5
    

    Use

    Cloning vector

    Tags

    V5

    Mutation

    Human codon optimized

    Promoter

    V5

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-human TCR zeta/2470

    Plasmid

    #11507

    Depositor

    Allan Weissman

    Article

    Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

    Insert

    TCR zeta (CD247 Human)
    

    Use

    General cloning

    Mutation

    n/a

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid

    #121722

    Purpose

    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1-P2A-AID (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc (C-term on osTIR1)

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 HCoV-OC43 NSP1_nostop

    Plasmid

    #168687

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    NSP1 (ORF1ab )
    

    Use

    Other

    Available Since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pXPE

    Plasmid

    #15284

    Depositor

    Ron Vale

    Article

    Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

    Insert

    ubiquitous kinesin heavy chain (KIF5B Human)
    

    Expression

    Bacterial

    Mutation

    The insert is missing the 1st 180 bp of 5' u…

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pDONR223 MERS-CoV NSP15_stop

    Plasmid

    #168640

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    NSP15 (orf1ab )
    

    Use

    Other

    Available Since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGEXNKI-his-GST-3C-LIC

    Plasmid

    #108709

    Purpose

    Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    GST-6xHis-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 Cas9-POLD3

    Plasmid

    #183197

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-POLD3
    

    Use

    CRISPR; Gateway entry clone

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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