We narrowed to 5,405 results for: dre

1. pMX-STAT5A-DN(-351)-Neo

   Plasmid

   #83249

   Purpose

   Retroviral expression of STAT5A dominant negative C-terminal deletion

   Depositor

   Andrew Brooks

   Insert

   Stat5A (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   C-terminal deletion of 351 nucleotides (dominant …

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNtCp-rbcL-PHLS-aadA-accD

   Plasmid

   #52470

   Purpose

   Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum

   Depositor

   Anastasios Melis

   Insert

   rbcL-PHLS-aadA-accD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_HCK

   Plasmid

   #224239

   Purpose

   Expression of human HCK Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   hck (HCK Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAMW-Wnk

   Plasmid

   #203978

   Purpose

   Myc6 Wnk under actin promoter for cell culture

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   6xMyc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

   Plasmid

   #50627

   Purpose

   pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

   Plasmid

   #50624

   Purpose

   pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Mrna production

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHZ018

   Plasmid

   #27504

   Depositor

   Andrew Fire

   Article

   Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

   Insert

   lin-4 (lin-4 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-RNAse A

   Plasmid

   #165565

   Purpose

   Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   RNAse A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pATetO 6XHis T5 Exonuclease

   Plasmid

   #206209

   Purpose

   Inducible expression of T5 exonuclease

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

   Insert

   T5 exonuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQR

    Plasmid

    #48099

    Purpose

    Cumate-inducible expression of mCherry-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQFT

    Plasmid

    #203674

    Purpose

    pQF derivative with promoter PQ5 exchanged for promoter PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    PQJ

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOpen-ETSSB

    Plasmid

    #165549

    Purpose

    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Extreme Thermostable Single-Stranded DNA Binding Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

    Plasmid

    #32628

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Sce
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. N1-Mito-pHCountdown

    Plasmid

    #182303

    Purpose

    mammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    mitochondria targeted pH countdown
    

    Use

    Tags

    COX8A mitochondria targeting sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKAR2-Br512g3.1

    Plasmid

    #171200

    Purpose

    Expresses Br512g3.1 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al medRxiv 2022.08.09.22278605

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (vHP47…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-N-HA

    Plasmid

    #119756

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCambia-loxKan

    Plasmid

    #199590

    Purpose

    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility

    Depositor

    Andreas Stuermer

    Insert

    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    

    Use

    Cre/Lox

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Nos

    Available since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pOpen-BsupolLF

    Plasmid

    #165563

    Purpose

    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Bsu DNA Polymerase I, Large Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pQC

    Plasmid

    #48100

    Purpose

    Cumate-inducible expression of ECFP-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    3xFLAG and ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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