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pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Publication

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRDA221 no Puro

Plasmid

#255841

Purpose

Backbone vector for expression of pspCas13b gRNA with EGFP

Depositor

Sefi Rosenbluh

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-WT

Plasmid

#252260

Purpose

Express HA-RHOA(WT)

Depositor

David Chan

Publication

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Available since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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PBgg-Puro

Plasmid

#248967

Purpose

Golden Gate assembly-friendly PiggyBac backbone with Puromycin resistance

Depositor

James Briscoe

Publication

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD2.0

Plasmid

#220536

Purpose

AAVS1-targeting vector designed for the iPS2-seq platform to express a single-copy, tetracycline-inducible shRNA in human pluripotent stem cells

Depositor

Alessandro Bertero

Publication

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Publication

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Publication

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Publication

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.puro-sh-γ-tubulin

Plasmid

#205602

Purpose

Tet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviral

Depositor

Tarun Kapoor

Publication

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

sh-g-Tubulin

Expression

Mammalian

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK2-puro

Plasmid

#199636

Purpose

sgRNA guide against VRK2

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK2 Human)

Use

Lentiviral

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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sgLacZ-puro

Plasmid

#199635

Purpose

sgRNA control guide targeting bacterial LacZ

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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sgCTRL-puro

Plasmid

#199634

Purpose

sgRNA control guide targeting intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pLentiguide-puro_hNKD2sg#3

Plasmid

#174325

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Publication

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#2

Plasmid

#174324

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Publication

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#1

Plasmid

#174323

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Publication

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_stgRNA-Puro

Plasmid

#164124

Purpose

Backbone for lentiviral stgRNA library construction (library 1 and 2)

Depositor

Hyongbum Kim

Publication

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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