We narrowed to 25,030 results for: Nes

1. pDGB3_omega2R

   Plasmid

   #68241

   Purpose

   GoldenBraid level 1 Omega plant expression vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Nano-lantern(Ca2+)_63/pRSETB

   Plasmid

   #51980

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-4GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Khc73(1-387)-GFP

   Plasmid

   #31747

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Khc73 (Khc-73 Fly)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Contains only aa 1-387

   Promoter

   Metallothionein

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

4. Khc73(1-432)-GFP

   Plasmid

   #31748

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Khc73 (Khc-73 Fly)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Contains only aa 1-432

   Promoter

   Metallothionein

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pEN_TTmiRC2_3xflag_Luciferase

   Plasmid

   #136519

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   Luciferase
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK412

   Plasmid

   #155371

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK430

   Plasmid

   #155377

   Purpose

   PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A2NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-PG13-mCherry-EF1a-PuroR-EGFP

   Plasmid

   #195292

   Purpose

   piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

   Depositor

   Hideyuki Oguro

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   mCherry
   PuroR-P2A-EGFP
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN_TT 3xFLAG-TWIST2

    Plasmid

    #197935

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGPTVII-Bar-U-MatryoshCaMP6s

    Plasmid

    #100024

    Purpose

    Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JD150

    Plasmid

    #229485

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, E4orf6, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-nlsABACUS2-400n

    Plasmid

    #203726

    Purpose

    Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge