We narrowed to 9,001 results for: ef1;

1. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMX-Gal4-TEAD4

   Plasmid

   #33105

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

   Insert

   TEAD4 (TEAD4 Human)
   

   Use

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAID6

   Plasmid

   #136989

   Purpose

   Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Inserts

   dLbCpf1-VP
   dSpCas9-RD1152
   SaCas9
   Csy4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO2, TDH3, TEF1, and TPI1

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDual-eGFP

   Plasmid

   #63215

   Purpose

   Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

6. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPXL-Sox9

   Plasmid

   #36979

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-BFP

   Plasmid

   #138272

   Purpose

   Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BFP H66 PAM
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H66, PAM motif

   Promoter

   Ef1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Integrin alpha5-AHCys

    Plasmid

    #27313

    Depositor

    Timothy Springer

    Article

    Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    integrin alpha5 (1-954) + AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. alpha7nACHR_Nacho_mCherry

    Plasmid

    #182939

    Purpose

    alpha7 nACHR expression in mammalian cell lines

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Synthesized

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_Stargazin-mTurquoise2-iLID

    Plasmid

    #161001

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Stargazin-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

    Plasmid

    #125696

    Purpose

    Global labeling of inhibitory synapses (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    mRuby2-Gephyrin.FingR-IL2RGTC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR V5-RNF25_IRES-mCherry

    Plasmid

    #198386

    Purpose

    RNF25 expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RNF25 (RNF25 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. E[beta]C

    Plasmid

    #24312

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

    Plasmid

    #132406

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLuc-CDS

    Plasmid

    #42100

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-ON VIPER CAR (anti-CD19)-final design

    Plasmid

    #192117

    Purpose

    Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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