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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 3941 - 3960 of 6562 results

  1. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid
    #198539
    Purpose
    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198539-plasmid-map-sequence-id-388890

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  3. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid
    #84245
    Purpose
    Expresses direct fusion KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    KRAB-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    84245-plasmid-map-sequence-id-160888

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  8. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid
    #134470
    Purpose
    Human expression vector for SaCas9-HF variant.
    Depositor
    Zongli Zheng
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    Use
    CRISPR
    Tags
    SV40NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    R245A, N413A, N419A, R654A
    Promoter
    CAG
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134470-plasmid-map-sequence-id-264583

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  9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    84247-plasmid-map-sequence-id-160890

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  12. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

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  15. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)

    Viral prep
    #192552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Guoping Feng
    Promoter
    CAG
    Tags
    tdTomato
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. ABE8.20-d

    Plasmid
    #136301
    Purpose
    expresses ABE8.20-d in mammalian cells
    Depositor
    Nicole Gaudelli
    Publication
    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8.20-d
    Tags
    C-terminal BPNLS
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    136301-plasmid-map-sequence-id-271476

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  18. MCP-M-MLV RT∆RNase H

    Plasmid
    #213749
    Purpose
    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213749-plasmid-map-sequence-id-422192

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Showing: 3941 - 3960 of 6562 results