We narrowed to 6,241 results for: nls

1. pMpGWB116

   Plasmid

   #68570

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Nuclear localization signal-tagged tdTomato (tdTo…

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMZ-176

   Plasmid

   #195424

   Purpose

   Transient, constitutive expression of synZiFTR: ZF1-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT121_GalL_nCDA1-VQRBE3

   Plasmid

   #145086

   Purpose

   Expressing base editor nCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-VQRBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ-347

   Plasmid

   #195458

   Purpose

   Lentiviral Integratable, constitutive expression of synZiFTR: ZF10-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - 3XFLAG - NLS - ZF1 - p65
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT222

   Plasmid

   #40942

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE2-VRER

   Plasmid

   #159984

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-VRER
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM122: pAAV-EFS-CasRx

   Plasmid

   #166870

   Purpose

   AAV vector for expressing CasRx

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.0_nucNanobody IRES EGFP

   Plasmid

   #136619

   Purpose

   Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-EnhancerNb-NLS (IRES EGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. epB_UbC_tdMS2cp-tagRFP-Tx2

    Plasmid

    #119908

    Purpose

    ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    HA-tdMS2cp-tagRFP-Tx2-nls
    

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT9748-dCBE

    Plasmid

    #163008

    Purpose

    plasmid expressing CBE with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ154

    Plasmid

    #69305

    Purpose

    Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9 (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Mutation

    NLS fusion

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMZ-178

    Plasmid

    #195426

    Purpose

    Transient, constitutive expression of synZiFTR: ZF3-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMZ-185

    Plasmid

    #195433

    Purpose

    Transient, constitutive expression of synZiFTR: ZF10-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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