We narrowed to 9,800 results for: otos

1. Thy-C73A-HA

   Plasmid

   #111542

   Purpose

   For expression of human KCNB1 in mouse brain

   Depositor

   Federico Sesti

   Article

   Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

   Insert

   thy1.2-C73A-HA (KCNB1 Human)
   

   Use

   Mouse Targeting

   Tags

   HA

   Expression

   Mutation

   C73A

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRG-2.1T-neo-sgMYB(human)#4.9

   Plasmid

   #105591

   Purpose

   lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   gRNA targeting human MYB DBD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG-2.1T-neo-sgMYB(human)#5.2

   Plasmid

   #105592

   Purpose

   lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   gRNA targeting human MYB DBD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSM4

   Plasmid

   #101642

   Purpose

   Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   KCyG4219
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   C-terminal residues (221-223) deletion. Ser220Val…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RSV/mGATA3 (antisense)

   Plasmid

   #83817

   Purpose

   contains antisense mouse GATA3

   Depositor

   Douglas Engel

   Article

   Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

   Insert

   GATA3-antisense strand (Gata3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

   Plasmid

   #70195

   Purpose

   Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR-deGFP fusion
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 213-petDuet1-GBD-LUMP--cdc42

   Plasmid

   #65973

   Purpose

   Dual expression of GBD-LUMP and cdc42

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Inserts

   LUMP
   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   GBD (GTPase binding domain from ACK1; residues 44…

   Expression

   Bacterial

   Mutation

   constitutively active Cdc42 (Q61L)

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-FLAG-WRAP53beta-siRNA-resistant

   Plasmid

   #64680

   Purpose

   expresses siRNA resistant WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

   Plasmid

   #60404

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMKO.1-puro/hFOXH1 shRNA6

    Plasmid

    #59310

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C

    Plasmid

    #60398

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptide

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid

    #60405

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid

    #58490

    Purpose

    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Glutamine

    Promoter

    Ubiquitin

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE159 GFP-delta LZ-XDpr-delta PDZB-CS2+

    Plasmid

    #16763

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    delta LZ-XDpr-delta PDZB (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Expression

    Mutation

    XDpr that is doubly truncated to delete the first…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pET28aAviHisCD72aCTLD

    Plasmid

    #129065

    Purpose

    generation of biotinylated mouse CD72a CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Article

    Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

    Insert

    CD72a (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-hUL

    Plasmid

    #27080

    Purpose

    Integration-free (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    L-MYC (LIN28A, MYCL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZac2.1-gfaABC1D-PinkyCaMP

    Plasmid

    #232859

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-mGold2s-Calnexin-N-14

    Plasmid

    #231768

    Purpose

    Visualization of ER in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Calnexin-N-17 (CANX Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRY2-mCherry-Miro1TM

    Plasmid

    #102247

    Purpose

    Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Use

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. GFP-Rab35 S22N inactive

    Plasmid

    #47426

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 S22N (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S22N inactive

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP_Rab35 Q67L active

    Plasmid

    #47425

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 Q67L (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Q67L active

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEX307-SARS-CoV-2-3CL

    Plasmid

    #160278

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cbh_v5 AAV-CBE C-terminal

    Plasmid

    #137176

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge