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Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB301
Plasmid#68629PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRU293
Plasmid#167688PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165887PurposeG418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRA99
Plasmid#209334PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega1
Plasmid#165864PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF279
Plasmid#167674PurposeIntermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGAPi
Plasmid#127547PurposeGateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segmentDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046740
Plasmid#145588PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2_171
Plasmid#199103PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046768
Plasmid#145763PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK506
Plasmid#155382PurposedCas12a (D917A) + PA4-mVenus. (removed crRNAs)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIN-VS1
Plasmid#197783PurposeLevel 2 destination vector: pMIN-VS1 backboneDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Omega 2
Plasmid#118047PurposeGoldenBraid2.0 Omega 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialMutationPromoterfimSAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only