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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,240 results for: codon optimized

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Showing: 3961 - 3980 of 5240 results

  1. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  5. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  7. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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  18. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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Showing: 3961 - 3980 of 5240 results