We narrowed to 17,501 results for: puro

1. pLV Rnf121-V5

   Plasmid

   #175130

   Purpose

   Lentiviral expression of mouse Rnf121-V5

   Depositor

   Larry Gerace

   Insert

   Rnf121 (Rnf121 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV Nup62-V5

   Plasmid

   #175116

   Purpose

   Lentiviral expression of mouse Nup62-V5

   Depositor

   Larry Gerace

   Insert

   Nup62 (Nup62 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. nuc-mCherry-pTRIP

   Plasmid

   #163520

   Purpose

   Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   nucleus targeted mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_PGK_Prkaa1

   Plasmid

   #204356

   Purpose

   Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter

   Depositor

   Günter Schneider

   Insert

   AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV Tor1a-V5

   Plasmid

   #175135

   Purpose

   Lentiviral expression of Tor1a-Myc

   Depositor

   Larry Gerace

   Insert

   Tor1a (Tor1a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV V5-Mospd1

   Plasmid

   #175132

   Purpose

   Lentiviral expression of V5-tagged mouse Mospd1

   Depositor

   Larry Gerace

   Insert

   Mospd1 (Mospd1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV Tyw1-V5

   Plasmid

   #175131

   Purpose

   Lentiviral expression of mouse Tyw1-V5

   Depositor

   Larry Gerace

   Insert

   Tyw1 (Tyw1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. StarShip01

    Plasmid

    #164505

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. StarShip02

    Plasmid

    #164506

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. StarShip03

    Plasmid

    #164507

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROP-seq-copGFP-v2-no-scaffold

    Plasmid

    #228520

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-PIG-N3FLAG-TAF12(50-130)

    Plasmid

    #105595

    Purpose

    retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    truncation fragments containing aa (50-130), and …

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Barcoded self-reporting transposon calling card collection

    Plasmid Kit

    #1000000213

    Purpose

    'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.

    Depositor

    Rob Mitra

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

19. CRISPaint Gene Tagging Kit

    Plasmid Kit

    #1000000086

    Purpose

    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

    Depositor

    Veit Hornung

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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