We narrowed to 41 results for: ligase labeling

1. pLX307-VHL-3F-BirA

   Plasmid

   #220147

   Purpose

   Expresses VHL-BirA for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-A3-HA-ubiquitin

   Plasmid

   #220145

   Purpose

   Expresses A3-Ub for E3 substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   A3-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEHAC1-HA-BirA-GSGS-Traf6

   Plasmid

   #232586

   Purpose

   Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   BirA -TRAF6 (TRAF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LAP2-beta-actin in modified pEGFP

   Plasmid

   #34839

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LAP2-beta-Actin (ACTB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pTM-BPL

   Plasmid

   #173766

   Purpose

   Fluorescent labeling of the nuclear envelope

   Depositor

   Shinji Sueda

   Article

   Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

   Insert

   PDGF receptor TM domain, Biotin protein ligase
   

   Tags

   FLAG and Signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX307-VHL(C162F)-3F-BirA

   Plasmid

   #220149

   Purpose

   Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed cysteine 162 to phenylalanine; codon opti…

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX307-CRBN-3F-BirA

   Plasmid

   #220146

   Purpose

   Expresses CRBN-BirA for CRBN substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX307-VHL(Y98H)-3F-BirA

   Plasmid

   #220148

   Purpose

   Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed tyrosine 98 to histidine; codon optimized

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSBbi_RP_MicroID2-HA

   Plasmid

   #188521

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbi_RP_lbMicroID2-HA

    Plasmid

    #188522

    Purpose

    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    lbMicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid

    #188523

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    3XNLS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid

    #188524

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    2XMTS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBbi_RP_MicroID2-FLAG-NES

    Plasmid

    #188525

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DTX2

    Plasmid

    #228447

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    DTX2 (DTX2 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RNF146

    Plasmid

    #228448

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    RNF146 (RNF146 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 8R6A

    Plasmid

    #228446

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    DTX1 (DTX1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEntr_Myc-BirA-G3-ER

    Plasmid

    #173814

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Use

    Gateway shuttle vector

    Tags

    Myc

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUAS-Myc-BirA-G3-ER

    Plasmid

    #173815

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Tags

    Myc

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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